Glycopeptide hexapeptides

ABSTRACT

The present invention is directed to glycopeptides and more particularly to derivatives of the glycopeptide A82846B. In these derivatives, the leucyl has been removed to create &#34;hexapeptides&#34; of A82846B and its N DISACC  variations. These hexapeptides are useful as antibacterials and also as starting materials from which further antibacterial compounds are prepared.

This application claims priority to provisional application Ser. No. 60/047,165 filed May 20, 1997.

BRIEF SUMMARY OF THE INVENTION

The present invention is directed to glycopeptides and more particularly to derivatives of the glycopeptide A82846B. In these derivatives, the leucyl has been removed to create "hexapeptides" of A82846B and its N^(DISACC) variations. These hexapeptides are useful as antibacterials and also as starting materials from which further antibacterial compounds are prepared.

DETAILED DESCRIPTION OF THE INVENTION

The present invention is directed to compounds of the formula ##STR1## wherein R¹ represents hydrogen or and epivancosaminyly radical of the formula ##STR2## wherein R² represents hydrogen or --CH₂ --R³ ; and R³ represents

alkyl of C₁ -C₁₁,

alkyl of C₁ -C₁₁ --R⁴, or

R⁴ --(0.sub.(0 or 1) --R⁴)₀ or 1,

wherein each R⁴ is independently phenyl or phenyl substituted by one or two substitutents, each of which is independently halo, loweralkyl of C₁ -C₄, loweralkoxy of C₁ -C₄, or loweralkylthio of C₁ -C₄, and pharmaceutically acceptable salts thereof.

The compounds of the present invention are prepared by an Edman degradation of a parent glycopeptide of the formula ##STR3## wherein R² is as defined above. The Edman degradation is a two-step process for the cleavage of the N-terminal residue of a peptide or protein. In the present invention, the above parent glycopeptide is first reacted with an isothiocyanate of the formula SCN-R⁵, to obtain an intermediate N^(LEU) -(thiocarbamoyl)-A82846B compound of the formula ##STR4## In the foregoing formula, R⁵ represents alkyl of C₁ -₁₀,

phenyl,

naphthyl, or

phenyl substituted by one or two substituents, each of which is independently halo, loweralkyl of C₁ -C₄, loweralkoxy of C₁ -C₄, benzyloxy, nitro, or ##STR5## wherein each R⁶ is independently loweralkyl of C₁ -C₄.

This reaction is conveniently carried out in water with pyridine, at a temperature of 25° to 30° C., employing a slight excess of the isothiocyanate reactant. The N^(LEU) -(thiocarbamoyl)A82846B intermediate can be separated in conventional manner or can be employed after removal of reaction solvent in the second step of the Edman degradation.

In the second step, the N^(LEU) -(thiocarbamoyl)A82846B is reacted with an organic acid, preferably trifluoroacetic acid, in a non-polar solvent such as dichloromethane. The reaction proceeds at temperatures of from 0° C. to 35° C. but is preferably carried out at temperatures of from 0° C. to 25° C. The reaction is generally complete in several hours. The resulting hexapeptide product is separated and purified if desired in conventional procedures. When it is desired to employ a salt, the product is reacted in standard procedures.

This second step of the Edman degradation can in some instances result in loss of the disaccharide epivancosamine. Longer reaction times can be used to prepare the N^(DISACC) -des-epivancosaminyl (R¹ =hydrogen) compound of the present invention.

The following examples illustrate the preparation of the compounds of the present invention.

Preparation of N^(DISACC) -(p-(p-chlorophenyl)benzyl)-N^(LEU) -(phenylthiocarbamoyl)A82846B

N^(DISACC) -(p-(p-Chlorophenyl)benzyl)A82846B trihydrochloride (100.0 mg, 0.0526 mmol) was dissolved in 10 ml H₂ O-pyridine (1:1 v/v) and treated with phenyl isothiocyanate (0.010 ml, 0.083 mmol). The resulting mixture was stirred at room temperature for 1 hr at which time HPLC analysis indicated complete consumption of the starting material. The reaction mixture was concentrated in vacuo and the crude product was purified by preparative HPLC to give 76.6 mg (76% yield) of N^(DISACC) -(p-(p-chlorophenyl)benzyl)-N^(LEU) -(phenylthiocarbamoyl)A82846B. FAB-MS: calc. For C₉₃ H₁₀₂ Cl₃ N₁₁ O₂₆ S 1925.5, obtained 1928.5 (M+3).

Preparation of Compound of Example 6, from Isolated Thiourea

A sample of purified N^(DISACC) -(p-(p-chlorophenyl)benzyl)-N^(LEU) -(phenylthiocarbamoyl)A82846B (63.3 mg, 0.0327 mmol) was suspended in 10 ml CH₂ Cl₂, cooled to 0° C., then treated with trifluoroacetic acid (0.10 ml). After 1 hr the reaction mixture was warmed to room temperature and stirred an additional 2 hr. The solvent was removed in vacuo and the crude product was purified by preparative HPLC to give 25.3 mg (46% yield) of N^(DISACC) -(p-(p-chlorophenyl)benzyl)desleucyl-A82846B as a white powder. FAB-MS: calc. for C₇₉ H₈₄ Cl₃ N₉ O₂₅ 1663.5, obtained 1666.4 (M+3).

Preparation of Compound of Example 4, from Parent Antibiotic without Isolation of Thiourea Intermediate N^(DISACC) -(p-Phenylbenzyl)A82846B (41.0 mg, 0.0233 mmol) was dissolved in 4 ml H₂ O-pyridine (1:1 v/v) and treated with phenyl isothiocyanate (0.0040 ml, 0.033 mmol). The resulting mixture was stirred at room temperature for 3 hr at which time HPLC analysis indicated complete consumption of the starting material. The reaction mixture was concentrated in vacuo to give the crude thiourea intermediate as a white solid. The thiourea derivative was then suspended in 10 ml CH₂ Cl₂, cooled to 0° C., then treated with trifluoroacetic acid (0.25 ml). After 30 minutes the reaction mixture was warmed to room temperature and stirred an additional 1 hr. The solvent was removed in vacuo and the crude product was purified by preparative HPLC to give 14.0 mg (37% yield) of N^(DISACC) -(p-phenylbenzyl)desleucyl-A82846B as a white powder. FAB-MS: calc. for C₇₉ H₈₅ Cl₂ N₉ O₂₅ 1629.5, obtained 1632.5 (M+3).

The HPLC procedures reported in these examples were as follows:

Analytical: Reactions were monitored by analytical HPLC using a Waters C₁₈ μBondapak or Novapak C₁₈ column (3.9×300 mm) and UV detection at 280 nm. Elution was accomplished with a linear gradient of 5% CH₃ CN-95% buffer to 80% CH₃ CN-20% buffer over 30 minutes. The buffer used was 0.5% triethylamine in water, adjusted to pH 3 with H₃ PO₄.

Preparative: Crude reaction mixtures were purified by preparative HPLC using a Waters C₁₈ Nova-Pak column (40×300 mm) and UV detection at 280 nm. Elution was accomplished with a linear gradient of 5% CH₃ CN--95% buffer to 80% CH₃ CN--20% buffer over 30 minutes. The buffer used was 0.5% triethylamine in water, adjusted to pH 3 with H₃ PO₄. The desired fractions were subsequently desalted with a Waters C₁₈ Sep-Pak (35 cc) followed by lyophilization.

Compounds were desalted as follows. A Waters Sep-Pak cartridge was pre-wet with methanol (2-3 column volumes) then conditioned with water (2-3 column volumes). The sample, dissolved in a minimum volume of water, was loaded onto the Sep-Pak column which was then washed with water (2-3 column volumes) to remove the unwanted salts. The product was then eluted with an appropriate solvent system, typically 1:1 CH₃ CN/H₂ O, CH₃ CN, and/or methanol. The organic solvent component was removed in vacuo and the resulting aqueous solution lyophilized to give the final product.

Representative compounds of the present invention are listed in the following table.

                                      TABLE I     __________________________________________________________________________                                    Analytical                                    HPLC,     Ex #         Name              FAB-MS                                M + X                                    min     __________________________________________________________________________     1   DESLEUCYL-A82846B 1466.7                                3    4.6*     2   N.sup.DISACC -(p-CHLOROBENZYL)-                           1590.5                                3   14.3*         DESLEUCYL-A82846B     3   N.sup.DISACC -(p-PHENOXYBENZYL)-                           1648.3                                3   17.4*         DESLEUCYL-A82846B     4   N.sup.DISACC -(p-PHENYLBENZYL)-                           1632.5                                3   17.1*         DESLEUCYL-A82846B     5   N.sup.DISACC -(p-n-BUTYLBENZYL)-                           1612.5                                3    13.6**         DESLEUCYL-A82846B     6   N.sup.DISACC -(p-(p-CHLOROPHENYL)-                           1666.4                                3    14.2**         BENZYL)DESLEUCYL-A82846B     7   N.sup.DISACC -DES-EPIVANCOAMINYL-                           1323.4                                2    8.5**         DESLEUCYL-A82846B     8   N.sup.DISACC -(8-PHENYL-n-                           1654.6                                3    16.1**         OCTYL)DESLEUCYL-A82846B     __________________________________________________________________________      *Waters C.sub.18 NOVA column      **Waters C.sub.18 μBondapak

The compounds of the present invention are useful for the treatment of bacterial infections. Therefore, in another embodiment, the present invention is directed to a method for controlling a bacterial infection in a host animal, typically a warm-blooded animal, which comprises administering to the host animal an effective, antibacterial amount of a compound of the present invention. In this embodiment, the compounds can be used to control and treat infections due to various bacteria, but especially gram-positive positive bacteria. In a preferred embodiment, the compounds are used to control and treat infections due to bacteria resistant to existing antibacterials. For example, certain bacteria are resistant to methicillin, and yet others are resistant to vancomycin and/or teicoplanin. The present compounds provide a technique for controlling and treating infections due to such resistant bacterial species.

In carrying out this embodiment of the invention, the compounds of the present invention can be administered by any of the conventional techniques, including the oral route and parenteral routes such as intravenous and intramuscular. The amount of compound to be employed is not critical and will vary depending on the particular compound employed, the route of administration, the severity of the infection, the interval between dosings, and other factors known to those skilled in the art. In general, a dose of from about 0.5 to about 100 mg/kg will be effective; and in many situations, lesser doses of from about 0.5 to about 50 mg/kg will be effective. A compound of the present invention can be administered in a single dose, but in the known manner of antibacterial therapy, a compound of the present invention is typically administered repeatedly over a period of time, such as a matter of days or weeks, to ensure control of the bacterial infection.

Also in accordance with known antibacterial therapy, a compound of the present invention is typically formulated for convenient delivery of the requisite dose. Therefore, in another embodiment, the present invention is directed to a pharmaceutical formulation comprising a compound of the present invention, in combination with a pharmaceutically- acceptable carrier. Such carriers are well known for both oral and parenteral routes of delivery. In general, a formulation will comprise a compound of the present invention in a concentration of from about 0.1 to about 90% by weight, and often from about 1.0 to about 3%.

The antibacterial efficacy of the present compounds is illustrated by the table. The minimal inhibitory concentrations (MICs) were determined using a standard broth micro-dilution assay.

                                      TABLE II     __________________________________________________________________________     Antibacterial Activity, Minimal Inhibitory     Concentration (MIC) against Various Organisms*     Ex           SA SA SA SH SH SE SPY SPN     #  Resistant             Sensitive                  446                     489                        447                           105                              415                                 270                                    C203                                        P1     __________________________________________________________________________     1  >128 >128 64 32  64                            >64                               >64                                  64                                     4     2  >81  5.2  4  0.5                        2  2  4  2  ≦0.06     3  45   14   2  1  1  2  1  0.5                                    ≦0.06                                        ≦.06     4  16   3.4  2  0.25                        1  1  1  1  ≦0.06                                        0.125     5  108  9.2  1  1  2  1  2  1  0.125                                        0.25     6  6.7  2.6  2  2  1  0.5                              1  0.5                                    ≦.06                                        0.25     7  >128 56   32 8  64 64 >64                                 >64                                    4   2     8  5.7  0.76 4  1  2  0.25                              2  1  0.125                                        0.125     __________________________________________________________________________     *ABBREVIATIONS               ORGANISM     RESISTANT Enterococcus faecium and faecalis               (geometric mean of 4-6 isolates)     SENSITIVE Enterococcus faecium and faecalis               (geometric mean of 4-6 isolates)     SA446     Staphylococcus aureus 446     SA489     Staphylococcus aureus 489     SA447     Staphylococcus aureus 447     SH 105    Staphylococcus haemolyticus 105     SH 415    Staphylococcus haemolyticus 415     SE 270    Staphylococcus epidermidis 270     SPY C203  Streptococcus pyogenes C203     SPN P1    Streptococcus pneumoniae P1

The compounds of the present invention can also be employed as starting materials to other antibacterial compounds. More particularly, the present hexapeptides can be reacted to introduce an alkyl group on the free "N¹ " amine. Alkylation is achieved by reacting the hexapeptide with an aldehyde to form a Schiff's base, which is then reduced to obtain the N¹ -alkylhexapeptide. Both reactions are carried out in a polar solvent, such as DMF, and at temperatures of 0-100° C., preferably 60-70° C. The preferred reducing agent is sodium cyanoborohydride. In one embodiment, the reducing agent is added at the same time as the hexapeptide and aldehyde. The resulting N¹ -alkylated hexapeptides are useful as antibacterials and can be employed as described above for the present compounds. 

We claim:
 1. A compound of the formula ##STR6## wherein R¹ represents hydrogen an epivancosaminyl radical of the formula ##STR7## wherein R² represents --CH₂ --R³ ; and wherein R³ representsalkyl of C₁ -C₁₁, alkyl of C₁ -C₁₁ --R⁴, R⁴ --(0.sub.(0 or 1) --R⁴)₀ or 1,wherein each R⁴ is independently phenyl or phenyl substituted by one or two substituents, each of which is independently halo, loweralkyl of C₁ -C₄, loweralkoxy of C₁ -C₄, or loweralkylthio of C₁ -C₄, or a pharmaceutically accetable salt thereof.
 2. A compound of claim 1 in which R¹ is epivancosaminyl wherein R² is hydrogen.
 3. A compound of claim 1 in which R¹ is epivancosaminyl wherein R² is --CH₂ --R³ as defined.
 4. A compound of claim 3 in which --CH₂ --R³ is p-(p-chlorophenyl)benzyl.
 5. A pharmaceutical formulation comprising a compound of claim 1 in combination with a pharmaceutically-acceptable diluent or carrier.
 6. A method of treating a bacterial infection in a host comprising the step of administering to the host an effective amount of a formulation of claim
 5. 7. A method of claim 6 wherein the bacterial infection is attributable to a vancomycin-resistant-enterococcus.
 8. A process for the preparation of a compound as claimed in claim 1 which comprises treating a compound of the formula ##STR8## wherein R² is as defined in claims 1-4, and R⁵ represents alkyl of C₁ -C₁₀,phenyl, naphthyl, or phenyl substituted by one or two substituents,each of which is independently halo, loweralkyl of C₁ -C₄, loweralkoxy of C₁ -C₄, benzyloxy, nitro, or ##STR9## wherein each R⁶ is independently loweralkyl of C₁ -C₄, with an organic acid in a non-polar solvent, and optionally forming a pharmaceutically acceptable salt. 